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Unlocking the Hidden Kinome

  • Michael T. McManus, Ph.D.
    Professor, University of California, San Francisco
    Vincent and Stella Coates Endowed Chair
    Director, UCSF Keck Center for Noncoding RNAs
    Core Director, UCSF Sandler Lentiviral RNAi Core
    Department of Microbiology and Immunology
    UCSF Diabetes Center


The McManus project uses Cas9-based technologies (CRISPRi, CRISPRa, and wtCRISPR) to interrogate GPCR, NR, ion channel, and kinase genes whose perturbation can cooperate or interfere with signal transduction, specifically cell proliferation, cell death, and differentiation. Dr. Michael T. McManus’ work for IDG is applying the CRISPR-CAS technology to unlock the hidden kinome (and will expand to other IDG protein classes) allowing for measuring the connection between genes and pathways by the utility of gene expression, gene activation or gene edition. Goals are to validate these Tdark matter and additional screens for epistatis.



Boettcher M, McManus MT. Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR. Mol Cell. 2015 May 21;58(4):575-85. doi: 10.1016/j.molcel.2015.04.028. PMCID: PMC4441801, PubMed Abstract:


Genes Under investigation, Michael T. McManus, Ph.D.

Submitted 8/2016

Contact: (415) 476-1114

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